I need help on finishing this assignment.. This is the question for the 2. and my answers are below.. The college sent these comments back .. can someone help me finish it correctly?
B. Explore how mitochondrial disease can occur at multiple levels in different mitochondrial processes by doing the following:
1. Explain what would hypothetically happen to the amount of ATP available to a cell if the entire Cori cycle were to occur and remain within a single cell (e.g., a muscle cell).
a. Identify specific numbers of ATP generated and used in the different parts of the cycle, in conjunction with your response from part B1.
2. Create an original dynamic diagram that shows how the citric acid cycle (CAC) is central to aerobic metabolism.
** These in bold are the ones I need help with.. ***
Note: A dynamic diagram is a clearly labeled diagram that uses arrows to indicate movement and interactions.
B1- If the Cori cycle were to be limited in the muscles, in case of low oxygen tension in the muscle cells, the cells will undergo anaerobic respiration which leads to the formation of lactic acid. When there is defect at any level of mitochondrial process, the lactic acid accumulated won’t be converted back to pyruvate hence hindering the process from proceeding to the Krebs’s cycle where more molecules ATP are produced. In this case, the process will be limited to glycolysis and the electron transport chain to generate ATP leading to a decrease in ATP production.
Identify specific numbers of ATP generated and used in the different parts of the cycle, in conjunction with your response from part B1.
B1A- The process will use two molecules of ATP in the following processes:
Phosphorylation of glucose to glucose-6-phosphate uses one molecule of ATP.
Phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate uses on molecule of ATP.
Because the process will only stop at the glycolytic pathway, the following amount of ATPs will be
produced at the energy payoff phase of glycolysis:
2 molecules of NADH are produced in the reaction involving oxidation of glyceraldehyde phosphate to 1,3-bisphosphoglycerate. The 2 molecules of NADH will enter the electron transfer chain reaction to produce 6 ATP.
2 molecules of ATP is produced in the substrate level phosphorylation reaction in which 1,3-bisphosphoglycerate to 3-phosphoglycerate.
2 molecules of ATP are produced in the dephosphorylation reaction of both PEP molecules to two pyruvates
In summary, the reaction will produce 10 ATP molecules.
B2. Create an original dynamic diagram that shows how the citric acid cycle (CAC) is central to aerobic metabolism.
Note: A dynamic diagram is a clearly labeled diagram that uses arrows to indicate movement and interactions.
ANSWER.
B1A-
Comments on this criterion: 2/7/16 – The submission correctly states that 2 ATP will be gained in the glycolysis portion of the Cori cycle. A description of the amounts of ATP generated or used in each part of the Cori cycle is not evident.
B2-
Comments on this criterion: 2/7/16 – An accurate, labeled diagram of the citric acid cycle is provided that shows the correct substrates, products and enzymes at each of the eight steps of the cycle. The diagram also shows how various metabolites produce Acetyl-CoA which enters the citric acid cycle. A diagram that shows how the citric acid cycle is central to aerobic metabolism is not evident.
