PROJECT DESCRIPTION
Does protein level affect the metabolic rate of zebrafish?
STATEMENT OF THE PROBLEM
dietary ingredients, nutrients as well as anti-nutritional factors are important factors that affect its growth and development. However, there is lack of proper nutritional control due to absence of standardized reference diet (Boyle 5354). Moreover, many epidemiological studies indicate that several prenatal and peri-natal dies are important in the growth and development of Zebra fish (Danio rerio) (Siccardi et al 23). In order to provide standardized dietary framework, there is need for the provision of specific dietary and nutritional standard to improve the growth and development of Zebra fish (Danio rerio). Thus, the need for investigating the growth and metabolic rate of Zebra fish (Danio rerio) when fed with different commercial diets.
BACKGROUND
How can protein affect the metabolic rate?
Does the oxygen level in the water indicate the metabolic rate?
STUDY SPECIES ( Zebra Fish)
Zebra fish (Danio rerio) is a small shoaling fish and it belongs to the minnow family of Cyprinadae and order cypriniformes. Due to its unique characteristics, the Zebra fish (Danio rerio) is mostly used as a vertebrae model organism in scientific research. It has regenerative qualities and has been modified by various researchers to produce several transgenic strains. It has been used in various medical research studies and findings including those dealing with the prevention of cancer, cardio-vascular conditions as well as investigation of the immune systems and other drug discovery systems
Furthermore, due to their omnivores qualities, they are have been used in environmental monitoring activities in order to prevent water pollution by estrogen (Howard 1173). Based on previous literatures and research studies investigating their growth and metabolic rate, it is evident that the Zebra fish (Danio rerio) are imperative in studying the development, genetics as well as the human disease conditions (Williams et al 153).
HYPOTHESES, GOALS, AND OBJECTIVES
HYPOTHESES: Protein will increase the metabolic rate.
GOALS: To test the metabolic rate after using 6 different protein level in commercial diet by measuring the oxygen level
OBJECTIVES: ??
METHODS
I will use 6 commercial diet with different level of protein and the same level of fat and fibers
Group 1
|
Name |
protein |
Fat |
Fiber |
|
Nutrafin Max Flake Food |
44% |
5% |
2% |
|
Tetra Pond Koi Vibrance |
31% |
5% |
2% |
Group 2
|
Name |
Protein |
Fat |
Fiber |
|
TetraMin Tropical Flakes |
38% |
6% |
6% |
|
HBH Algae Grazers |
28% |
6% |
6% |
|
Name |
Protein |
Fat |
Fiber |
|
New Life Spectrum Premium |
48% |
5% |
4% |
|
Dainichi Veggie Deluxe |
28% |
5% |
4% |
I will use 3 tanks each tank have 2 male Zebra Fish. (Find the different between male and female metabolic rate) (Why did I choose male??)
I will measure the oxygen level by using
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Salifert Oxygen Test Kit |
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Quick and accurate test for dissolved oxygen content. Proper oxygen concentration is vital for aquarium inhabitants and the overall health of the aquarium environment. The Salifert Oxygen Profi Test allows extremely fast, professional measurement of dissolved oxygen. Maintaining proper oxygen levels is especially important in marine aquariums because of the low solubility of oxygen in saltwater. The Salifert Oxygen Test Kit is a “must-have” test kit for hobbyists with sophisticated aquarium devices such as calcium reactors, CO2 systems, or ozonizers, where regular monitoring of oxygen level is crucial. 50 tests. Use for freshwater or marine aquariums.
This is mainly because when the oxygen level decrease that means Fish are using more oxygen to increase their metabolic rate. Previous research studies and findings also indicate that some of the methods used in measuring the metabolic rate of animals include the double labeled water (DLW) and heart rate methods (HRM). The best method is the DLW since it is cheaper and provides best procedures to determine the standard errors in estimating oxygen consumption. However, the heart rate method is more accurate and provides reliable calibrations. In measuring the rate of metabolic rate of Zebra fish (Danio rerio) various commercial diets
A light:dark photoperiod of 12:12 h was provided by indirect fluorescent lighting. Temperature was monitored daily using a thermometer (Fisher Scientific, Pittsburgh, PA). Ammonia-nitrogen, NO2-N, and pH were monitored weekly using an aquarium pharmaceuticals test kit (Aquarium Pharmaceuticals, Chalfon, PA). Each tank contained one cylindrical plastic mesh cage (0.25 m height by 0.09 m diameter) covered with nylon mesh (Fashion Knee Hi; American Corporation, Henderson, NC). Temperature was maintained at 28°C (±0.5°C), pH ranged between 8.0 and 8.4, and ammonia-nitrogen and NO2-N remained below visual detection limits.
