Practice and understand transcription and translation at the Utah genetics website before doing the homework. Use codon table provided in class for protein synthesis. http://learn.genetics.utah.edu/content/begin/dna/transcribe/ Given the DNA sequence below:
3’-CATCGTCCTTCTATACTTCGCGGATACCCGGTCCATGTCGGTTCACATCCACGT-5’
1. Replicate the strand by writing the complementary sequence. Label the 5’ and 3’ ends in the new strand. 2. Assume the original strand is the template strand for a gene. Transcribe the template strand to an mRNA sequence. Label the 5’ and 3’ end of the mRNA. 3. Find the start codon on the mRNA and enclose it in a box. 4. Write the amino acid sequence of the translation product starting from the start codon, until you encounter a stop codon. Enclose the stop codon with a box. If a stop codon is not present, create one by adding nucleotide sequences to both the gene and mRNA. 5. During DNA replication, a mutation occurred and GGT in the sequence given above, got deleted from the gene. Transcribe the mutated DNA strand. Give the amino acid sequence of the resulting mutated protein. If a stop codon is not present, create one by adding sequences to the gene and mRNA. 6. In another mistake in DNA replication, a T (indicate in yellow color) was incorporated in place of the C. Transcribe the mutated DNA strand, then determine the amino acid sequence of the mutant protein.
